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|Title: ||Insights from next-generation proteomics of bacterial endophytes response to chickpea root exudates and GacS role on plant growth-promoting traits regulation|
|Authors: ||Brígido, Clarisse|
Torres, Denise P.
|Keywords: ||legume-rhizobia symbioses|
plant growth promotion
|Issue Date: ||5-Oct-2021|
|Citation: ||Brígido C; Paço A.; Torres, D. P.; Steinmetz G.; Armengaud J. (2021) Insights from next-generation proteomics of bacterial endophytes response to chickpea root exudates and GacS role on plant growth-promoting traits regulation. XXX Reunión Latinoamericana de Rizobiología y la V Conferencia Latinoamericana de Microorganismos Promotores del Crecimiento Vegetal, 4-8 October 2021, Uruguay (online).|
|Abstract: ||Besides the beneficial association that legumes establish with rhizobia, these plants are also colonized by other endophytic bacteria. Although it
is believed that these bacteria also have an important role on plant fitness, the
molecular mechanisms of how non-rhizobial bacteria respond to the host-derived signals are poorly understood compared to the well-characterized N2
legumes-rhizobia symbioses. Here, a high-throughput proteomic analyses of two
endophytic bacteria detected proteins involved in metabolism, cell envelope biosynthetic process, stress response, defense against oxidative stress, chemotaxis,
nitrogen metabolic process, type iv secretion system and transmembrane transport as response to chickpea root exudates. One of the genes highly upregulated
in cellular proteome of Pseudomonas sp. Q1 after exposition to chickpea root
exudates is the gene on locus 4453. This gene (2,754 bp) encodes the GacS (global
activator of antibiotic and cyanide synthesis), a membrane-bound sensor hybrid
histidine kinase, member of the Gac two-component signal transduction system.
To investigate the phenotypic traits under the regulation of the Pseudomonas sp.
Q1 GacS protein, a knockout mutant for gacS gene was obtained. The swarming
and swimming motility, biofilm production or phosphate solubilization were not
affected by gacS gene deletion on ∆gacS mutant strain. On the other hand, the
production and secretion of siderophores by ∆gacS mutant was reduced around
8.8% compared with the wild-type strain but the biosynthesis of antimicrobial
metabolites in vitro was not affected. Our results showed that GacS of Q1 strain
is only partially involved in the regulation of the mainly plant beneficial traits of
Pseudomonas sp. Q1, suggesting that in this strain the role of this protein can be
fulfilled by one or more additional two-component signal transduction systems.
However, further studies are necessary to clarify the role of GacS in the regulation
of surface motility, siderophore production and antifungal activity of Q1 strain.|
|Appears in Collections:||MED - Comunicações - Em Congressos Científicos Internacionais|
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