Please use this identifier to cite or link to this item:

Title: The root lesion nematode effector Ppen10370 is essential for parasitism of Pratylenchus penetrans
Authors: Vieira, Paulo
Vicente, Claudia
Branco, Jordana
Buchan, Gary
Mota, Manuel
Nemchinov, Lev
Keywords: Pratylenchus penetrans
Issue Date: 12-Apr-2021
Publisher: Molecular Plant-Microbe interactions
Citation: Vieira P, Vicente CSL, Branco J, Buchan G, Mota M, Nemchinov LG. The Root Lesion Nematode Effector Ppen10370 Is Essential for Parasitism of Pratylenchus penetrans. Mol Plant Microbe Interact. 2021 Jun;34(6):645-657.
Abstract: The root lesion nematode, Pratylenchus penetrans, is a migratory species that attacks a broad range of crops. Like other plant pathogens, P. penetrans deploys a battery of secreted protein effectors to manipulate plant hosts and induce disease. Although several candidate effectors of P. penetrans have been identified, detailed mechanisms of their functions and particularly their host targets remain largely unexplored. In this study, a repertoire of candidate genes encoding pioneer effectors of P. penetrans was amplified from mixed life stages of the nematode, cloned and subjected to transient expression in a heterologous host Nicotiana benthamiana using potato virus X-based gene vector. Among seven analyzed genes, the candidate effector designated as Ppen10370 triggered pleiotropic phenotypes substantially different from those produced by wild type infection. Transcriptome analysis of plants expressing Ppen10370 demonstrated that observed phenotypic changes were likely related to disruption of core biological processes in the plant due to effector-originated activities. Cross-species comparative analysis of Ppen10370 identified homologue gene sequences in five other Pratylenchus species and their transcripts were found to be localized specifically in the nematode esophageal glands by in situ hybridization. RNA silencing of the Ppen10370 resulted in a significant reduction of nematode reproduction and development, demonstrating an important role of the esophageal gland effector for parasitism.
Type: article
Appears in Collections:MED - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica

Files in This Item:

File Description SizeFormat
2021_Vieira, Vicente et al..pdf2.41 MBAdobe PDFView/Open
FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpaceOrkut
Formato BibTex mendeley Endnote Logotipo do DeGóis 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.


Dspace Dspace
DSpace Software, version 1.6.2 Copyright © 2002-2008 MIT and Hewlett-Packard - Feedback
UEvora B-On Curriculum DeGois