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Title: Identification and characterization of the first pectin methylesterase gene discovered in the root lesion nematode Pratylenchus penetrans
Authors: Vicente, Cláudia
Keywords: Pratylenchus penetrans
Pectin methyl esterase
Issue Date: 2019
Publisher: PLoS
Citation: Vicente, C.S.L., L.G. Nemchinov, M. Mota, J.D. Eisenback, K. Kamo, and P. Vieira. 2019. Identification and characterization of the first pectin methylesterase gene found in the root lesion nematode Pratylenchus penetrans. PLoS One. 2019 Feb 22;14(2):e0212540. doi: 10.1371/journal.pone.0212540.
Abstract: Similar to other plant-parasitic nematodes, root lesion nematodes possess an array of enzymes that are involved in the degradation of the plant cell wall. Here we report the identification of a gene encoding a cell wall-degrading enzyme, pectin methylesterase PME (EC, in the root lesion nematode Pratylenchus penetrans. Both genomic and coding sequences of the gene were cloned for this species, that included the presence of four introns which eliminated a possible contamination from bacteria. Expression of the Pp-pme gene was localized in the esophageal glands of P. penetrans as determined by in situ hybridization. Temporal expression of Pp-pme in planta was validated at early time points of infection. The possible function and activity of the gene were assessed by transient expression of Pp-pme in plants of Nicotiana benthamiana plants via a Potato virus X-based vector. To our knowledge, this is the first report on identification and characterization of a PME gene within the phylum Nematoda.
Type: article
Appears in Collections:MED - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica

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