Please use this identifier to cite or link to this item: http://hdl.handle.net/10174/2627

Title: Isolation of total RNA from ripe and unripe soursop (Annona muricata L.) fruit
Authors: Brasil, Isabella Montenegro
Otoch, Maria de Lourdes Oliveira
Costa, José Hélio
Maia, Geraldo Arraes
Lima, Maria da Guia Silva
Arnholdt-Schmitt, Birgit
Fernandes de Melo4, Dirce
Keywords: Annona muricata L.
RNA isolation
tropical fruit
Issue Date: Sep-2008
Publisher: Academic Journals
Abstract: Soursop fruit tissue is known by its acidic pH and high levels of polysaccharides, polyphenolics and secondary metabolites. These conditions are recognized to interfere unfavorably with conventional methodologies for RNA isolation. We describe here a rapid and simple method for the isolation of total RNA from soursop fruit. RNA was extracted in less than 4 h through a combination of SDS/potassium acetate precipitation and selective binding on a silica-gel-based membrane (Qiagen) through microspin speed technology. In comparison to other methods applied for RNA extraction from soursop fruit, our protocol improved substantially RNA quality as well as RNA yield. The isolated RNA served as a robust template for RT-PCR analysis. Comparable RNA quality and yield per dry weight were obtained from unripe and ripe fruits. This makes the method appropriate to being used in studies on differential gene expression in post-harvest behavior.
URI: http://hdl.handle.net/10174/2627
ISSN: 1996-0824 (online)
Type: article
Appears in Collections:ICAAM - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica

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