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Title: MIRU-VNTR typing adds discriminatory value to groups of Mycobacterium bovis and Mycobacterium caprae strains defined by spoligotyping
Authors: Duarte, Elsa Leclerc
Domingos, Margarida
Amado, Alice
Cunha, M
Botelho, Ana
Keywords: Mycobacterium bovis
Bovine tuberculosis
Issue Date: Jul-2010
Publisher: Elsevier
Abstract: The value of Mycobacterial Interspersed Repetitive Units-Variable Number Tandem Repeats (MIRU-VNTR) as a genotyping technique for Mycobacterium bovis and Mycobacterium caprae, has been confirmed in different countries and epidemiological scenarios. However, a standardized panel of loci has not yet been adopted for these species, since allelic diversity of each locus differs among countries. To determine the most discriminatory loci, a panel of 181 M. bovis and M. caprae strains representing 12 spoligotypes was created. The panel included strains from the three predominant spoligotypes previously isolated in Portugal; strains from spoligotyping group SB0140, prevalent in the British Isles but also detected in Portugal; strains from spoligotypes common to cattle and wildlife species and strains from the M. caprae spoligotyping group SB0157. MIRU-VNTR analysis of these strains, targeting 8 selected loci, produced 87 different profiles (h = 0.99), being VNTR3232, QUB11a, ETR-B and ETR-A the most discriminatory loci (h = 0.96). A single M. bovis spoligotyping group could be differentiated – up to 44 MIRU-VNTR profiles. These results emphasize the high genotype diversity of Portuguese isolates compared with other countries. MIRU-VNTR typing was superior to spoligotyping for identifying multi-genotype infected herds and the combination of the two genotyping methods by a hierarchical approach confirmed the genetic relatedness of M. bovis isolates between cattle and wildlife.
Type: article
Appears in Collections:MVT - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica
MED - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica

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