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|Title: ||Quantification of free auxins in semi-hardwood plant cuttings and microshoots by dispersive liquid–liquid microextraction/microwave derivatization and GC/MS analysis|
|Authors: ||Pofírio, S.|
da Silva, M. G.
Cabrita, M. J.
|Issue Date: ||11-Jul-2016|
|Publisher: ||The Royal Society of Chimestry|
|Citation: ||Porfírio, S., Sonon, R., da Silva, M.G., Peixe, A., Cabrita, M.J., Azadi, P., (2016) Quantification of free auxins in semi-hardwood plant cuttings and microshoots by dispersive liquid–liquid microextraction/microwave derivatization and GC/MS analysis. Analytical Methods, 31:8, 6089-6098|
|Abstract: ||Several studies have suggested that differences in the natural rooting ability of plant cuttings could be
attributed to differences in endogenous auxin levels. Hence, during rooting experiments, it is important
to be able to routinely monitor the evolution of endogenous levels of plant hormones. This work reports
the development of a new method for the quantification of free auxins in auxin-treated Olea europaea
(L.) explants, using dispersive liquid–liquid microextraction (DLLME) and microwave assisted
derivatization (MAD) followed by gas chromatography/mass spectrometry (GC/MS) analysis. Linear
ranges of 0.5–500 ng mL 1 and 1–500 mg mL 1 were used for the quantification of indole-3-acetic acid
(IAA) and indole-3-butyric acid (IBA), respectively. Determined by serial dilutions, the limits of detection
(LOD) and quantification (LOQ) were 0.05 ng mL 1 and 0.25 ng mL 1, respectively for both compounds.
When using the calibration curve for determination, the LOQ corresponded to 0.5 ng mL 1 (IAA) and
0.5 mg mL 1 (IBA). The proposed method proved to be substantially faster than other alternatives, and
allowed free auxin quantification in real samples of semi-hardwood cuttings and microshoots of two
olive cultivars. The concentrations found in the analyzed samples are in the range of 0.131–0.342 mg g 1
(IAA) and 20–264 mg g 1 (IBA).|
|Appears in Collections:||FIT - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica|
MED - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica
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