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Title: Decline in peroxidase and catalases by lindane may cause an increase in reactive oxygen species in Saccharomyces cerevisiae
Authors: Pita, T
Alves-Pereira, I
Ferreira, R
Editors: Méndez-Vilas, A
Keywords: organochlorine
gluthatione peroxidase
Issue Date: 2014
Publisher: Wageningen Academic Publishers
Citation: Pita T, Alves-Pereira I, Ferreira R (2014) Decline in peroxidase and catalases by lindane may cause an increase in reactive oxygen species in Saccharomyces cerevisiae, In: A. Méndez-Vilas (ed), Industrial, medical and environmental applications of microorganisms: Current status and trends, 1sd ed, Wageningen Academic Publishers, Wageningen, NL, ISBN Print version: 978-90-8686-243-6, ISBN E-book: 978-90-8686-795-0, p. 83-87
Abstract: Lindane is an organochlorine insecticide, persistent in soils and aquifers, lipophilic, chemically and biochemically inert that accumulates along the human food chain. It is commonly used on a wide variety of crops, in warehouses, in public health to control insect-borne diseases, and as a seed treatment. Lindane is also presently used in lotions, creams, and shampoos for the control of lice and mites in humans. Several chemicals as lindane, toxic for aquatic organisms, birds and mammals have been in the news recently, since the European Union intends to ban it. Therefore it is urgent to clarify the toxicological mechanisms of this compound in eukaryotic cells. Thus the main purpose of this work was to evaluate the effects of lindane in the wine wild-type Saccharomyces cerevisiae UE-ME3. The results show that lindane inhibited cell growth of S. cerevisiae UE-ME3, causing a decrease in the biomass produced along 72 h, as well as cell viability from 24 h of assay. On the other hand, was detected an increase in the reactive oxygen species content of post-12,000 g sediment of cells exposed to 5 µM lindane and post-12000 g supernatant of cells subjected to any exposure conditions, eventually conditioned by a decline in glutathione peroxidesase and catalase T activities, which has become the detoxification of hydrogen peroxide less effective. The increase in the CAT A activity without significant changes in the alkaline phosphatase and Se-GPx activities justified, in part, the increase in ROS levels of S. cerevisiae exposed to lindane, as well as the loss of cell viability due to inadequate response of glutathione cycle or cells signaling pathways that assure lipid biosynthesis.
ISBN: 978-90-8686-243-6
Type: bookPart
Appears in Collections:QUI - Publicações - Capítulos de Livros
MED - Publicações - Capítulos de Livros

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