Please use this identifier to cite or link to this item: http://hdl.handle.net/10174/7152

Title: Involvement of alternative oxidase (AOX) in adventitious rooting of Olea europaea L. microshoots is linked to adaptive phenylpropanoid and lignin metabolism
Authors: Santos Macedo, E.
Sircar, D.
Cardoso, H.G.
Peixe, A
Arnholdt-Schmitt, B
Editors: Hahne, G.
Liu, J.R.
Keywords: Alternative oxidase
Flavonoids
Lignin
Olea europaea
Salicylhydroxamic acid
Total phenolics
Issue Date: Sep-2012
Publisher: Springer-Verlag
Citation: Santos Macedo, E., Sircar, D., Cardoso, H.G., Peixe, A., Arnholdt-Schmitt, B. Involvement of alternative oxidase (AOX) in adventitious rooting of Olea europaea L. microshoots is linked to adaptive phenylpropanoid and lignin metabolism. Plant Cell Rep.,31(9):1581-1590
Abstract: Alternative oxidase (AOX) has been proposed as a functional marker candidate in a number of events involving cell differentiation, including rooting efficiency in semi-hardwood shoot cuttings of olive (Olea europaea L.). To ascertain the general importance of AOX in olive rooting, the auxin-induced rooting process was studied in an in vitro system for microshoot propagation. Inhibition of AOX by salicylhydroxamic acid (SHAM) significantly reduced rooting efficiency. However, the inhibitor failed to exhibit any effect on the preceding calli stage. This makes the system appropriate for distinguishing dedifferentiation and de novo differentiation during root induction. Metabolite analyses of microshoots showed that total phenolics, total flavonoids and lignin contents were significantly reduced upon SHAM treatment. It was concluded that the influence of alternative respiration on root formation was associated to adaptive phenylpropanoid and lignin metabolism. Transcript profiles of two olive AOX genes (OeAOX1a and OeAOX2) were examined during the process of auxin-induced root induction. Both genes displayed stable transcript accumulation in semi-quantitative RT-PCR analysis during all experimental stages. In contrary, when the reverse primer for OeAOX2 was designed from the 3’ -UTR instead of the ORF, differential transcript accumulation was observed suggesting posttranscriptional regulation of OeAOX2 during metabolic acclimation. These result confirms former observations in olive semi-hardwood shoot cuttings on differential OeAOX2 expression during root induction. It further points to the importance of future studies on the functional role of sequence and length polymorphisms in the 3’ -UTR of this gene. Key message The manuscript reports the general importance of AOXin olive adventitious rooting and the association of alternative respiration to adaptive phenylpropanoid and lignin metabolism.
URI: http://hdl.handle.net/10174/7152
Type: article
Appears in Collections:FIT - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica
ICAAM - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica

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