Please use this identifier to cite or link to this item:
http://hdl.handle.net/10174/39076
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| Title: | The potential of enzymatic hydrolysis with HS-SPME-GC×GC-ToFMS for the study of volatile signature of grapes |
| Authors: | Fonseca, Daniela
Martins, Nuno
Garcia, Raquel
Cabrita, Maria João |
| Keywords: | Aroma composition
HS-SPME-GC×GC-ToFMS
AR2000 enzyme
Clones |
| Issue Date: | 2025 |
| Publisher: | Elsevier - LWT |
| Citation: | Daniela Fonseca, Nuno Martins, Raquel Garcia, Maria João Cabrita,
The potential of enzymatic hydrolysis with HS-SPME-GC×GC-ToFMS for the study of volatile signature of grapes,
LWT,
Volume 215,
2025,
117314,
ISSN 0023-6438,
https://doi.org/10.1016/j.lwt.2024.117314. |
| Abstract: | This works focuses on developing an optimized methodology for analyzing free and bound volatile compounds in grapes using enzymatic hydrolysis with AR2000 enzyme and HS-SPME-GC × GC-ToFMS. To achieve this purpose, a previous optimization step of enzymatic hydrolysis was implemented and optimal conditions for enzymatic hydrolysis were established using 50 mg of AR2000 enzyme at 35 °C for 24 h, while the best extraction conditions were found to be 40 min at 60 °C. Applying this methodology to clones of Moreto grapes from harvest of 2022 and 2023, it was possible to identify and semi-quantify sixty volatile compounds, with more compounds identifiable in 2022 than in 2023. The results also showed the influence of crop year and genetic intravariability. |
| URI: | http://hdl.handle.net/10174/39076 |
| Type: | article |
| Appears in Collections: | MED - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica
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MED - Instituto Mediterrâneo para Agricultura, Ambiente e Desenvolvimento /
MED - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica /
