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Title: Exposure to titanium dioxide nanoparticles in heat-shock conditions reverses glucose-induced fermentation of Saccharomyces cerevisiae
Authors: Capela-Pires, J
Ferreira, R
Alves-Pereira, I
Keywords: yeast
aerobic fermentation
cell viability
Issue Date: 2017
Publisher: FORMATEX - Research Center
Citation: Capela-Pires, Joana, Ferreira, Rui and Alves-Pereira, Isabel (2017) Exposure to titanium dioxide nanoparticles in heat-shock conditions reverses glucose-induced fermentation of Saccharomyces cerevisiae, Biomicroworld 2017, Madrid, Espanha.
Abstract: The consume of glucose by alcoholic fermentation in Saccharomyces cerevisiae involves a step of decarboxylation of pyruvate to acetaldehyde, catalysed by the enzyme pyruvate decarboxylase (PDC) that is followed by the reduction of acetaldehyde to ethanol by the enzyme alcohol dehydrogenase (ADH). In general S. cerevisiae uses the aerobic alcoholic fermentation to oxidize NADH in NAD+, generated by the glycolytic pathway, when glucose is available as carbon source. The aim of this study was: (a) to induce the aerobic alcoholic fermentation in the S. cerevisiae UE-ME3 grown in YP medium with glycerol (4%) (YPG) by the addition of glucose (2%) (YPGD), and (b) to evaluate a possible modulating effect on this metabolic change by the heat shock or TiO2-NP exposure in heat shock conditions. Titanium dioxide nanoparticles (TiO2-NP) with molecular size less than 100 nm were added to the culture of S. cerevisiae UE-ME3 100 min after the addition of glucose, maintaining the agitation conditions (180 rpm) and the temperature at 28 ºC or still applying heat shock (28/40 ºC) (HS) by raising the temperature of the culture from 28 ºC to 40 ºC. The reading and discussion of the results included the evaluation of the influence of culture conditions on cell viability (cfu) and on the fermentative metabolism of S. cerevisiae, at level of the enzyme activities pyruvate decarboxylase (PDC) and alcohol dehydrogenase (ADH). The addition of glucose to the YPG medium caused an increase in the cell viability and enzyme markers of alcoholic fermentation (PDC and ADH) of Saccharomyces cerevisiae. After this first step it was assessed whether the heat shock (28/40ºC) or the yeast cells exposure to TiO2-NP <100 nm (5 g/mL) in heat shock conditions or not, for 100 min was able to reverse this effect. S. cerevisiae exposed to heat shock (28/40 °C) in the last 100 min of culture exhibited cell viability and level of PDC activity close to those detected in cells grown in YPG medium. However, S. cerevisiae in heat-shock conditions exhibited a decrease in ADH activity to the levels lower than those detected in yeasts cells grown in the control media (YPG and YPGD). The exposure of S. cerevisiae to TiO2-NP <100 nm, at 100 min of growth in YPGD media caused a decrease in cell viability to levels close to those determined in yeast cells grown only in YPG medium. Interestingly, S. cerevisiae exposed in the last 100 min of the culture to TiO2-NP <100 (5 μg/ml), in heat shock (28/40 °C) conditions, exhibited cell viability, PDC and ADH enzyme activities levels closed to those determined in yeast cells grown only in YPG medium. The decrease in cell viability and the slowdown of the alcoholic fermentation in aerobic conditions caused by the simultaneous exposure of S. cerevisiae UE-ME3 to TiO2-NP <100 nm (5 μg/mL) and heat-shock (28/40), after 100 min of culture in glucose, points to a negative modulation of the aerobic alcoholic fermentation in this wine wild-type yeast from Alentejo, Portugal.
Type: lecture
Appears in Collections:MED - Comunicações - Em Congressos Científicos Internacionais

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