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|Title: ||A real time Taqman RT-PCR for the detection of rabbit hemorrhagic disease virus 2 (RHDV2)|
|Authors: ||Duarte, Margarida Dias|
Carvalho, Carina L.
Barros, Silvia C.
Henriques, Ana M.
Duarte, Elsa L.
|Issue Date: ||2015|
|Citation: ||Duarte, Margarida Dias; Carvalho, Carina L.; Barros, Silvia C.; Henriques, Ana M.; Ramos, Fernanda; Fagulha, Teresa; Luís, Tiago; Duarte, Elsa L.; Fevereiro, Miguel. A real time Taqman RT-PCR for the detection of rabbit hemorrhagic disease virus 2 (RHDV2), Journal of Virological Methods, 219, 1, 90-95, 2015.|
|Abstract: ||As key prey, the wild rabbit downsize constitutes a major drawback on the endangered Iberian lynx (Lynx
pardinus) re-introduction in the Iberia. Several captive breeding units mostly located in Alentejo, endeavour the wild
rabbit repopulation of depleted areas assigned for the lynx re-introduction.
Here we report an RHDV2 outbreak that occurred in early 2016 in a wild rabbit captive breeding unit located
in Barrancos municipality. The estimated mortality rate between March and April 2016 was approximately 8.67%.
Anatomopathologic examination was carried out for 13 victimized rabbits. Molecular characterization was based on
the complete vp60 capsid gene.
The 13 rabbit carcasses investigated showed typical macroscopic RHD lesions testing positive to RHDV2-
RNA. Comparison of the vp60 nucleotide sequences obtained from two specimens with others publically available
disclosed similarities below 98.22% with RHDV2 strains originated in the Iberia and Azores and revealed that the two
identical strains from Barrancos-2016 contain six unique single synonymous nucleotide polymorphisms.
In the phylogenetic analysis performed, the Barrancos-2016 strains clustered apart from other known strains,
meaning they may represent new evolutionary RHDV2 lineages. No clear epidemiological link could be traced for
this outbreak where the mortalities were lower compared with previous years. Yet, network analysis suggested a
possible connection between the missing intermediates from which the strains from Barrancos 2013, 2014 and 2016
have derived. It is therefore possible that RHDV2 has circulated endemically in the region since 2012, with periodic
Still, six years after its emergence in wild rabbits, RHDV2 continues to pose difficulties to the establishment of
natural wild rabbit populations that are crucial for the self-sustainability of the local ecosystems.|
|Appears in Collections:||MVT - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica|
ICAAM - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica
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