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|Title: ||Variation of the group 5 grass pollen allergen content of airborne pollen in relation to geographic location and time in season|
|Authors: ||Buters, Jeroen|
|Issue Date: ||6-May-2015|
|Publisher: ||Journal of Allergy and Clinical Immunology|
|Citation: ||J. Buters, M. Prank, M. Sofiev, G. Pusch, R. Albertini, I. Annesi-Maesano, C. Antunes, H. Behrendt, U. Berger, R. Brandao, S. Celenk, C. Galan, L. Grewling, R. Kennedy, A.i Rantio-Lehtimäki, G. Reese, I. Sauliene, M. Smith, M. Thibaudon, B. Weber, L. Cecchi (2015). Airborne Phl p 5 in different fractions of ambient air deviates from grass pollen counts in 10 countries across Europe. J Allergy Clin Immunology 136(1): 87-95e6|
|Abstract: ||Background: Allergies to grass pollen are the number one cause
of outdoor hay fever. The human immune system reacts with
symptoms to allergen from pollen.
Objective: We investigated the natural variability in release of
the major group 5 allergen from grass pollen across Europe.
Methods: Airborne pollen and allergens were simultaneously
collected daily with a volumetric spore trap and a high-volume cascade impactor at 10 sites across Europe for 3 consecutive years.
Group 5 allergen levels were determined with a Phl p 5–specific
ELISA in 2 fractions of ambient air: particulate matter of greater
than10mmin diameter andparticulatematter greater than 2.5mm
and less than 10 mm in diameter. Mediator release by ambient air
was determined in FcεRI-humanized basophils. The origin of
pollen was modeled and condensed to pollen potency maps.
Results: On average, grass pollen released 2.3 pg of Phl p 5 per
pollen. Allergen release per pollen (potency) varied substantially,
ranging from less than 1 to 9 pg of Phl p 5 per pollen (5% to 95%
percentile). The main variation was locally day to day. Average
potency maps across Europe varied between years. Mediator
release from basophilic granulocytes correlated better with
allergen levels per cubic meter (r2 5 0.80, P < .001) than with
pollen grains per cubic meter (r2 5 0.61, P < .001). In addition,
pollen released different amounts of allergen in the non–pollenbearing
fraction of ambient air, depending on humidity.
Conclusion: Across Europe, the same amount of pollen released
substantially different amounts of group 5 grass pollen allergen.
This variation in allergen release is in addition to variations in
pollen counts. Molecular aerobiology (ie, determining allergen
in ambient air) might be a valuable addition to pollen counting.|
|Appears in Collections:||MED - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica|
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