Please use this identifier to cite or link to this item: http://hdl.handle.net/10174/2104

Title: A novel membrane-bound Ech [NiFe] hydrogenase in Desulfovibrio gigas
Authors: Rodrigues, Rute
Valente, Filipa
Pereira, Inês
Oliveira, Solange
Rodrigues-Pousada, Claudina
Keywords: hydrogenase
Desulfovibrio gigas
Issue Date: 2003
Abstract: Abstract: In the present study, we report the identification of an operon with six coding regions for a multisubunit membrane-bound [NiFe] hydrogenase in the genome of Desulfovibrio gigas. Sequence analysis of the deduced polypeptides reveals a high similarity to subunits of proteins belonging to the family of Ech hydrogenases. The operon is organised similarly to the operon coding for the Ech hydrogenase from Methanosarcina barkeri, suggesting that both encode very similar hydrogenases. Expression of the operon was detected by Northern blot and RT-PCR analyses, and the presence of the encoded proteins was examined by Western blotting. The possible role of this hydrogenase is discussed, relating it with a potential function in the H-2 cycling as a mechanism for energy conservation in D. gigas. The present study provides therefore valuable insights into the open question of the energy conserving mechanism in D. gigas
URI: http://hdl.handle.net/10174/2104
Type: article
Appears in Collections:ICAAM - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica
BIO - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica

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