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|Title: ||Biological and Molecular Characterization of Olive latent virus 1|
|Authors: ||Félix, M. Rosário|
Cardoso, Joana M.S.
Clara, M. Ivone E.
|Keywords: ||OLV-1 virus|
|Issue Date: ||2007|
|Abstract: ||Olive latent virus 1 (OLV-1) belongs to the Necrovirus genus, Tombusviridae family and is pathogenic to olive, citrus and tulip plants. It is
easily mechanically transmissible to indicator plants causing necrotic lesions and can be transmitted through the soil into the plant roots in
the absence of biological vectors. Infected cells contain virus aggregates, inclusions made up of excess of viral coded peptides and
extensive vesiculation in the cytoplasm. The virions are isometric with ca. 30 nm, possess a monopartite single-stranded positive-sense
RNA genome sized 3700 nt with 5 open reading frames (ORFs) and small inter cistronic regions. ORF 1 encodes a polypeptide with a
molecular weight of 23 kDa and the read through of its amber stop codon results in ORF 1 RT that encodes the virus RNA dependent
RNA polymerase with 82 kDa. ORF2 and ORF3 encode two small peptides, with 8 kDa and 6 kDa, respectively, which appear to be
involved in the virus cell-to-cell movement. ORF 4 is located in the 3′-terminal and encodes a protein with 30 kDa identified as the viral
coat protein. The complete genomic sequences of two well characterized OLV-1 isolates (obtained from citrus and olive) are similar,
revealing an overall nucleotide sequence identity of 95%. The electrophoretic profile of the dsRNAs recovered from infected tissues
exhibits three major species with ca. 3.7, 1.5, and 1.3 kbp. Application of molecular techniques based on PCR and on dot blot hybridization
has been successfully used for routine diagnosis of OLV-1 infections.|
|Appears in Collections:||ICAAM - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica|
BIO - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica
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