Please use this identifier to cite or link to this item: http://hdl.handle.net/10174/30678

Title: Castanea sativa mill. flowers as potential chemopreventive agent against rat prostate cancer model
Authors: Nascimento-Gonçalves, Elisabete
Silva, M
Seixas, Fernanda
Fardilha, Margarida
Ferreira, Rita
Neuparth, Maria João
Faustino-Rocha, Ana Isabel
Colaço, Bruno
Venâncio, Carlos
Rosa, Eduardo
Barros, L
Ferreira, Isabel CFR
Oliveira, MM
Peixoto, Francisco
Oliveira, Paula Alexandra
Issue Date: 2020
Publisher: L Reunião Sociedade Portuguesa de Farmacologia, XXXVIII Reunião Farmacologia Clínica, XIX Reunião Toxicologia
Citation: Nascimento-Gonçalves E, Silva M, Seixas F, Fardilha M, Ferreira R, Neuparth MJ, Faustino-Rocha AI, Colaço B, Venâncio C, Rosa E, Barros L, Ferreira ICFR, Oliveira MM, Peixoto F, Oliveira PA. 2020. Castanea sativa mill. flowers as potential chemopreventive agent against rat prostate cancer model. L Reunião Sociedade Portuguesa de Farmacologia, XXXVIII Reunião Farmacologia Clínica, XIX Reunião Toxicologia, 5 a 7 de fevereiro, Coimbra, Portugal.
Abstract: Introduction: Prostate cancer is one of the most common cancer among men, having a huge impact in their health [1]. This work aimed to evaluate the influence of a decoction extract obtained from C. sativa flowers (CF) on chemically and hormonally induced rat prostate cancer animal model. Material & Methods: All the animal experiments were approved by the Institutional Animals Ethics Committee and by Portuguese national authorities (DGAV no 021326). Forty male Wistar Unilever rats were randomly divided into four groups: control group (n=10), induced group (n=15), CF control group (n=5) and CF induced group (n=10). Animals from induced groups received a multistep induction protocol, which consisted of sequential administration of flutamide, testosterone propionate, the carcinogenic agent MNU and crystalline testosterone. The CF extract, rich in ellagitannins especially trigalloy-HHDP- glucose, was administered in the drinking water (3 mg/animal/day) for 49 weeks. Animals were sacrificed at 61 weeks of age and organs were collected, weighed and processed for light microscopy. Data were analysed using SPSS and GraphPad Prism software. Results: There were no significant differences in relative mean liver weight among groups exposed and not exposed to the CF extract and no animals developed severe hepatic changes. Animals from CF induced group developed less prostatic intraepithelial neoplasia than induced group. Also, animals exposed to the CF extract did not present areas of inflammation of the dorsolateral prostate lobe greater than 50% unlike the groups not exposed (p<0.05). The administration of CF in induced animals was able to decrease the activity of CAT and GST by 36% and 20%, respectively (p<0.05). Conclusions: These results suggest that CF extract was well tolerate by the animals and did not cause severe hepatic and renal toxicity. C. sativa flowers extract may be used as chemopreventive agent against prostate cancer and seems to have an antioxidant role.
URI: http://hdl.handle.net/10174/30678
Type: lecture
Appears in Collections:ZOO - Comunicações - Em Congressos Científicos Nacionais

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