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Title: A Novelty System for Biotization of Plant Microshoots and Collection of Natural Compounds
Authors: Castro, Mário Rui da Costa Basílio e
Ragonezi, Carla
Oliveira, Paulo Guilherme Leandro de
Zavattieri, Maria Amely
Keywords: biotechnology
chemical analysis
double-phase medium
phenolic compounds
liquid chromatography—diode array detector—mass spectrometry
Issue Date: 7-Jan-2019
Abstract: An in vitro plant microshoot culture system composed of two phases; a liquid phase overlaid by a floating solid phase, which is described in detail herein. This system is designed to enable the extraction of natural compounds released/disseminated into the liquid phase during root growth, thus facilitating their processing and biochemical characterization. The solid phase holds the plant afloat and enables the simultaneous culture of a microorganism, yet avoiding its penetration into the liquid phase, where the roots are submerged. Both phases can be independently formulated as required for growth optimization of both organisms. Considering the closed system and known variables described in this patent, applications of the described method include testing with pesticides, herbicides, and other similar products.
Type: article
Appears in Collections:ICT - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica
CIBIO-UE - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica

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