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|Title: ||A novel Rhodotorula sp. RNA-FISH probe for the identification of this artwork’s biodeterioration agent|
|Authors: ||Branco, Patrícia|
Caldeira, Ana Teresa
|Issue Date: ||17-May-2018|
|Publisher: ||Universidade de Évora|
|Citation: ||Branco, P.; Candeias; A.; Caldeira, A. T.; González-Pérez, M. A novel Rhodotorula sp. RNA-FISH probe for the identification of this artwork’s biodeterioration agent (2018). In Web of Knowledge: A look into the Past, embracing the Future, Universidade de Évora, Portugal. pp.74 .|
|Abstract: ||Thus, the aim of this study was to design in silico a novel RNA-FISH probe to Rhodotorula sp., that facilitate a precise identification of this yeast, and to evaluate its specificity and experimental performance. The probe designed was a novel 26S RNA probe whose experimental evaluation was done by constructing the fluorescence-signal-response/formamide concentration curve for the target and a non-target yeast (Cryptococcus adeliensis).
In silico and experimental results revealed that the Rhodotorula sp. probe is genus-specific. Hence, this study will contribute to an accurate identification of microorganisms involved in the biodeterioration process, advantageous for the selection of proper remediation strategies.|
|Appears in Collections:||HERCULES - Comunicações - Em Congressos Científicos Internacionais|
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