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|Title: ||Glucose evoked regulation of Na,K-ATPase activity in pancreatic beta-cells|
|Authors: ||Costa, A.C.R.|
Antunes, Célia M.
Morais, Júlio C.
Enzyme activity regulation
|Issue Date: ||Oct-2008|
|Abstract: ||Glucose is the most important physiological insulin secretagogue. However, the mechanism of glucose-induced insulin release is not fully understood. The current dogma states that, in pancreatic -cell, glucose metabolism leads to an increase of ATP/ADP ratio, closure of ATP-sensitive K+ channels, membrane depolarization, opening of the voltage-dependent Ca2+ channels and Ca2+ influx which triggers insulin exocytosis. However, the role of other electrogenic systems, namely ionic pumps, to these events remains essentially uninvestigated.
Na,K-ATPase, responsible for maintaining Na+ and K+ gradients across the plasma membrane, extrudes 3Na+ in exchange for 2K+, generating a net outward current; Thus changes in its activity may contribute to the ionic events regulating insulin secretion. Regulation of Na,K-ATPase activity by glucose remains unclear and controversial, and has never been determined in intact -cells.
The aim of this work was to develop a method to characterize Na,K-ATPase activity in intact -cells and to evaluate whether glucose contributes to its regulation.|
|Appears in Collections:||QUI - Comunicações - Em Congressos Científicos Nacionais|
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