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|Title: ||Characterizing Microbial Diversity and Damage in Mural Paintings|
|Authors: ||Rosado, T|
Candeias, A J
Caldeira, A T
|Keywords: ||biodeterioration and biodegradation|
|Issue Date: ||2014|
|Publisher: ||Cambridge University Press|
|Citation: ||Rosado T, Mirão J, Candeias A and Caldeira AT (2014) Characterizing Microbial Diversity and Damage in Mural Paintings. Microscopy and Microanalysis doi:10.1017/S1431927614013439.|
|Abstract: ||Mural paintings are some of the oldest and most important cultural expressions of mankind and play
an important role for the understanding of societies and civilizations. These cultural assets have high economic
and cultural value and therefore their degradation has social and economic impact. The present work presents a
novel microanalytical approach to understand the damages caused by microbial communities in mural paintings.
This comprises the characterization and identification of microbial diversity and evaluation of damage promoted
by their biological activity. Culture-dependent methods and DNA-based approaches like denaturing gradient gel
electrophoresis (DGGE) and pyrosequencing are important tools in the isolation and identification of the
microbial communities allowing characterization of the biota involved in the biodeterioration phenomena.
Raman microspectrometry, infrared spectrometry, and variable pressure scanning electron microscopy coupled
with energy-dispersive X-ray spectrometry are also useful tools for evaluation of the presence of microbial
contamination and detection of the alteration products resulting from metabolic activity of the microorganisms.
This study shows that the degradation status of mural paintings can be correlated to the presence of metabolically
|Appears in Collections:||CQE - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica|
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