Please use this identifier to cite or link to this item:
|Title: ||Revista de Ciências Agrárias - Comportamento celular e resposta antioxidante diferenciados de Saccharomyces cerevisiae and Saccaromyces chevalieri ao metavanadato de amónio|
|Other Titles: ||Different cellular behaviour and antioxidant response of Saccharomyces cerevisiae and Saccharomyces chevalieri growing in presence of ammonium metavanadate|
|Authors: ||Ferreira, Rui Manuel Alves|
Ferreira, Isabel Maria Simão Alves Pereira
|Keywords: ||Saccharomyces cerevisiae|
|Issue Date: ||Jan-2007|
|Abstract: ||The fermentation of wine is a complex microbiological process which requires yeast adaptation to stress conditions. In the cellular environment of aerobic organisms naturally reactive oxygen species (ROS) occurs as by-products of mitochondrial respiration. The higher reactivity of these chemical species could cause molecular damages that in several cases induce cellular death. In common physiological conditions or as response to oxidative stress, the cell can generate adapted responses which involve antioxidants mechanisms as glutathione reductase (GR; EC 220.127.116.11) and catalase T (CAT T; EC 18.104.22.168) and A (CAT A; EC 22.214.171.124) enzymes.
Vanadium, a heavy metal present in several pesticides could generate ROS changing the intracellular redox state and cause deleterious effects in yeasts exposed to higher levels of this element. The main objective of this work was to compare the effects of ammonium metavanadate (NH4VO3), a pentavalent salt of vanadium on cellular viability and GR, CAT T and CAT A activities of wine yeast Saccharomyces cerevisiae UE-ME3 and Saccharomyces chevalieri UE-ME1.
The results obtained show that S. chevalieri UE-ME1 has lower tolerance to NH4VO3 than S. cerevisiae UE-ME3, since S. chevalieri cultures do not survive to concentration values of ammonium metavanadate higher than 7,5 mM, whereas S. cerevisiae cells are still viable in the presence of 75 mM. S. chevalieri has an enzymatic activity lower than S. cerevisiae, although for both yeast species NH4VO3 could behave as oxidative stress inductor, causing a significant decrease of GR activity (P<0,01) and a significant increase of CAT A activity (P<0,01). The results show also an increase of CAT T activity in both yeast species, which can be interpreted as a protective response to oxidative stress. Differences on response to amonium metavanadate by both species of Saccharomyces could be partially justified by more efficient antioxidant systems in S. cerevisiae UE-ME3.|
|Appears in Collections:||QUI - Publicações - Artigos em Revistas Nacionais Com Arbitragem Científica|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.