%PDF-1.6
%
2 0 obj
<>
endobj
12 0 obj
<>stream
2016-01-22T15:18:33Z
Canon
2016-01-22T15:27:13Z
2016-01-22T15:27:13Z
uuid:ddf0ce50-4f41-4976-8e99-407f9722b151
uuid:4daed23e-c71f-41c7-821c-530634721bda
application/pdf
endstream
endobj
3 0 obj
<>
endobj
373 0 obj
<>
endobj
374 0 obj
<>
endobj
195 0 obj
<>
endobj
196 0 obj
<>
endobj
197 0 obj
<>
endobj
346 0 obj
<>
endobj
375 0 obj
<>
endobj
376 0 obj
<>
endobj
377 0 obj
<>
endobj
386 0 obj
<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI]/Subtype 395 0 R/XObject<>>>/Type/Page>>
endobj
387 0 obj
<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI]/Subtype 400 0 R/XObject<>>>/Type/Page>>
endobj
388 0 obj
<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI]/Subtype 405 0 R/XObject<>>>/Type/Page>>
endobj
389 0 obj
<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI]/Subtype 410 0 R/XObject<>>>/Type/Page>>
endobj
390 0 obj
<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI]/Subtype 415 0 R/XObject<>>>/Type/Page>>
endobj
457 0 obj
<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI]/Subtype 463 0 R/XObject<>>>/Type/Page>>
endobj
458 0 obj
<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI]/Subtype 468 0 R/XObject<>>>/Type/Page>>
endobj
470 0 obj
<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI]/Subtype 475 0 R/XObject<>>>/Type/Page>>
endobj
477 0 obj
<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI]/Subtype 482 0 R/XObject<>>>/Type/Page>>
endobj
482 0 obj
<>stream
-
nsrC/V
CH/IPTER4
Geruenel DrscusstoN
Mycosphaerella species is one of the main Eucalyptus pathogens. lnChapter 1 is presented a literature review of both fungi and host. Nevertheless,Mycosphaerella species previous records did not include complete informationabout collections sites, dates pnd hosts.
ln order to better understand the distribution and Mycosphaerella'srelative importance, a phylogenetic study using ITS gene region to clusterisolates from two young E. globulus plantation was accomplished. This workcontributed to the identification of two new species in Portugal.
ln addition to the moledular analysis presented in Chapter 2, the sampleswere characterized based on morphological methods. ln some species, inparticular M. vespa and M. molleriana, different ascospore germination paftemswere observed which showed that these species may not be synonyms like wasrefereed on previous works. These results emphasize the importance ofmethodologies conjugation in order to obtain a classification.
Also in Chapter 3 was established the first evaluation on Eucalyptsclones and families susceptibility to MLD, this data are extremely important forwood pulp enterprises.
This work was the first step to understand the real MLD on eucalyptssituation in Portugal. Although the species identification was not completed, themethodology was tested and set up. ln the future, is necessary a completesurvey extended to all important areas of eucalypt plantations; such as thedetermination of baniers hetween different species of Mycosphaerella,sequnce difierent regions of genome, and completed with pathogenicity tests.
I
68
endstream
endobj
483 0 obj
<>/Filter/CCITTFaxDecode/Height 3333/Length 43048/Name/Obj4/Subtype/Image/Type/XObject/Width 2370>>stream
4
(B!2(Tt7O)*0AشDptu*^F7SС1oZ.5/ ]i-]z߭KO{ݽ~_]~pyQx0ݏ]7\d}}Bu_izp>~Xuo
'"?X28>+*{2(jPuI
>D$df2Dl@6h8!G
AHa4Ӹ ;:wՅ!(|3ő2)GtYSFo/Hr{A@z#ǯ!&).GpS$nI-W;2fq7, QBudAU$9,DFց^!d!.Ô(}.k/۽ק_ݫ
lEk>^ǻM7PeM28OMa@ө6-"[B9
Έ(g# $v1#|ʱFD9`1PhZ @<G$B$(a0ޓjOz
?O_zk'O}~ߦuK!Vd? B 7ȣAjE;mRtoND@pxwIi
[,uղCr1u3}??|? @d
w){ p~߭?m6;TaHo)Htb?Pc:)
pE_;"A
߷29{k]g;ߛ9oik
ۺZ_c_K}jWxjXi6_(n{iE1E;{kiq_iiw
a^!G_
L*V/ &a{&D!F
FPC4٥DFM""""1jr@I`*IHga
3 d\L~[R,˰
RfPM-3@W\)N*iy-D#rzmK8D@`2RFp!Dz*HKL5b
|CO?,tͳ<GgA!-0i;Fc5Ge4T-4Ӱ
5N*!c(~`!A&fHD5U $馟_dHQI`rD`9V$$ińVNM^YȐBsvD8("fB ﵽkD -E"~=|r*.2\fbhE!4jMiGGK(&7=O*!Cg@B d e@L&; h=SZgDL8`tҺ`A_iOzk脌2/ uI7^:ZҮiz4oNYz!^MHNh (''UT_mKtԂ'ӸU^^D$4E"m0AkvJ-b^\9aUnBs" à|==t_i{?!?ߨ[i*!!#!DN"&z
H?OOVXkd[UГ}~?4iHf<̡<.xP>*z\e@gKZ~^N}-+O-&m8{ב;O/u1WzZS֘{_-`눎φֺ]@g]%dr8ghx#ֈ?Y$կgcOJCQsG5`_*D,Pk~4Ao`wK!'%كtOzm?roW+o|rxV$IDTW
]]/KuB
b!/܌zu-&+c8OAiQ]*0DΗHA}w?wtM/[ᅳ ~~JZI) I|/vt`8tֿ`GZjp߭ ?`4ZbiWV+!6^C?!ACkj {}a/48w_UZKMm5_%M C_{][NՄML{_m}"c7Lŏ T`E%ZVUҪՃ.$lq[~Mw_M== LN}I_k00Mu[M{M{]zi ph2<@XkaX5
0{U[Mm4Mmjj@&0R2)+i%v ;
ᦜ2)H*`DDD)1ֵUV[Ԙ;T&1=kHk
tl idH YM駠;
]2V+ʴ z&E L!!!zA5D0B#{QQQKt8_dkT֨seUlfhY-칝Gz^vT-ea!i߭9^IMiKiK3eR2*dT3*$'u(uzkju$䀦D]
h8OMu_2!.)G˅::C Az
OzFb֎0@&}iܐNŧ~KHw)\!8 0LhBC7Q+(P"4
i莎">C>dP`B.=-,4GAr'a<(Q Dx!&da9"5AXMtZݪ"[܋Ϧ@Z,k!5rL8HE.дAON3#
C%:>]"ΡD2pB!09zO ԅz!/ nnmt>@f`=BiiAAxOZ[[VBD%dQ#14x'ރ>O?z!u![N >ӋixMS]1R/6aɎlރzu}mH~p}Ӻ ._}d ԄȐ"D^v AAn_ׯy9>û_|G Քz]| }
=
Rmw_4}뭃߈=nBm"&4G'6F=M:SwC'
;!/8Ǣ'9A|5'
'W__MWRoxA~u?pr{kl7n[ݥX":<H##MO_܆$6.J~믐i
GzN3t9p__.ӿ_$O؏#^ N)OV^#x4?? Ovi~Z Po5lU/@J~8?DCh'Ot=Q!-"&ݴO״$99\NJ{_jկ83ַr GlEc~U]]=b.~7&Y!y|##߂1ׄݖ:Kצ/XC8_!-`@{Btڲ
&ֿ#=z馻ֻײ?
_OXU|u^볣{}?_|/z\5z8?oizv[M5ovvڭ]OM
0kë.[[_c!~Mk5 ô4 [Ka2<cq~T{
T5샿p;M4jE(KWG\'&WۆiΆm*ڧinV0 h0LDDDDEl[N;ՃOL""""(4UmXjLu^h;!a6Ah1r2;_&v
=w
T9L0W}ՆaYq\0AdPA)2D!ZAiCXء">Ѝ-Yskᯃ))ѼSxL!gdr.!ri-l [3̨3 4. )¦_eZڑ]XțOFHiE CXӸѬ:@G"܇nWޫD:
N#
#0BpaaPA_̔q-#PBB>) p8:!CB0B,&/s 8tєpDLPBTӐ\qk+VEr\LDtl*a! aL`qwtӺCE!Nї"y8AA` i=ZYșFJhȈsFt0Mhd3A4&hX&GiAHJptf@s9&`4A5P k?M?@ù2"{>5On$.F
a(C!9! uL@!hZ
4! zn脊!.KhD
B~R~џ'Ai4xMoMONӯ],9N
<7>?JL'
M:MU;ziޝG&dGHyd\<"rh7AWY꺽o뭄f4 ŦWizwR%E"P@ z?ONZ_uUWcڧtjvGr#|"ЉAz
pujX_G}R{
iQB^B=H:HAKIwڪ.z"'Q9.PO<$<&O^il+IեIz#}>% {x]:MSӥvޗ}w4;/ׯRbz.'v4{kG7DuMVuw Ҫ?Xd9&|L]DFu_!$.IN_};Lz8g@/ G?{_KV
JfQf!o
D~!$6' ^C_?|Nm%kKZM%8":[[=֟n~`iHsاޟyN7z][N l4JAɎU^ yZNdH>?~~{__uҰWֈY (g9P_k^+
Xݶ_mj/R4J(6=n[2r:>8v~d aC?ﰛ
kml"#aa'j4Ma;}URIS=i$J׆m/apqG_\0! [Ua0JڶQt^7kw
&4MJֽm/KX`QǿMn|7}aAL }dzȵ(B Ј鴛J] aaabiOm?*G0D*6 """=X0T0*k5AڶAd
>M4*־)v[M2[aCjaS"L 4""""#ꖚk
i;Rn
aՑka 4Xw[M-;V5L,0L8"JS apvC܍q*B""?VMae:ehA!DF"ʩ0[DDDmNJB#꺆4*qӌGtD*,JuRlgddB;4\'Gu/|A|إ88"?i':5'HXN P5¼s\KIKMb!t hCq&c&By@P~qф `30`ABJh&e`@i4 izvj$"iM?L'߄U^ߧi~T_BC/!s"vGF:i~n"C9z4*txMzl7HLr(菴"'AAA%7O\'KX>"sAH;#'O'kW`m߆
;_Om5܇!O_t6i]78}k#]
Sq8 G$5a/\GHalVhqC\O?륐 =GU?}n?O_ڥ~[oװ܃uop׆!!:Pfw!%{ȧF|& ~$Y!'GT;ΌPMz_z4 KiT~d3n߿aaP^ڰ9o]տ=Z^uX}04Cҵ0گiZ]P}58lw]{Dna|0+
{`ՆbVXk
vV!]1+wN׃b+خMM[ ,uvwiWzkikWûiH~a4Mzi4Oᦷ
5xd<\2%a4e4
DmAݠh>
;a80@4AFSi(2! GaN i`C'!DD{4BGT""""""?C4RUdy2iPд岨ݟgfZd#&%[-|2XN3_$2dz?Kd&vLEAu·[JdŞ8'z; ǘˊlh5OW͙Òapb
At0U:a
9CڪiaOMPpTE"H.fP.!hd3G6hCrCM5
M-^T::")! ds6AZ!vjtiiRB+kEZ*@N#89L#a^C;MJWTB>
DGv%Q渗yL8Px 4-;kJhy n9cC''! hD]OO[C5ӿ tDפ@$߳ Z^"YDZ8A?P_mH/~NuBDK?$}O꺤»ւuIǐ»OT=8d#u#H,t=ݭuMRVLuj~BAԉDu2&P 8;<ҿ{}Q0Qx?!GHȃB;D#D}"&dLg27H n n?N]7VwtZaoA0h?7AC?K]?_/Kl:
^S'I}\#װK [UKW^&M֫kӴ`W]|=?}7R]SmodrxoGJJEu n`#{y7AߥפBk@\kxi? z% s^ Baj}zOҏOI0JH.~_'륵ֶ~8.ρd@}|碁jkuVZ}HllD% o^KZZVJaX%2~ƻ
(K;]?Jk[լ0҆㯽 _/X_zoZ[_EGqhkaկkl/ivwݶkiv va`6={M4m~I+_05^_c~=kMIkd_k-K#ki1xkꪘMl*C`a+$68:}xUAaS 0@XaAVcy5vҿm6߇
NpL{Xa;NJ 13kv2$Za0Dt!ڦvAp0Bi7&S"RCj
aUf^,2t*h04j!̨?HܣK}Qi?@iZrd3/N[el|'DGUrv-"BFKԛGd'p_ɰ!D~ԉE_]vt?"1ֿ#cx\RO47 dB
RA># OP~@]
CΙkT?zZ7O׃C6iWUﵿJH6KDժk^èkKK`E%LbWO4=_mv_`\q_r ~A;aAWB~{wh?nu[I^Q68j C*;ؓ_:d~'O'/5°¶=ا){^lx@/NmIk-C[_ki7l0O*
oa+
v8vkݠՆN.G[m+Vب|W6?>@qO0T L^v&=_ႰQal/l[}5!dž,D5aU@h2a[|kid0CA5X```&x+5M-״4
sa]4A ]Ўۯ})xiᅲ#0*i#2)XcA[Ɏ"<0ULX`F]rgQH[[]Bۿk{v[[d[ U+A6ԁq﵆/
UA+ U`%lMA":!}RK5jMDDDDDDDDq-+[_ m4k*At}@oTA=@ĚZ2X-m #,G8o#rDPѠhEi_ԵRQ/.G̉jv<M5
*cǖK30jk_|od[L_~2P}<&_"R6ΙC3'99kUZ'e"ʁ3~\)9!ikKs)?_2!DGN)99C6 D-pyAߠTTk]fK"H#>(" a0!~RҪ"⒡ 'CghA2`
ii=4>Zp!FHyPgghCL&CT
0v#wHѩS": A ֍ja!' Aӽ~4N״BwN%r@E!:#E:/F a t%в
D<- Ni }d&d_龃k nHf O
B40N,'~M4Nk_O3"DE"~at7A~}WS UUN/ UT BAʋ\Bv֨=p'
i}~znwz~ڤ!-H#D82^ZA;ڠ`'W⯪z_OOUZ_D$hh`U AIWڴB=6װMiyA!#!,0q2"K} 7{5r
;__"/>ȘvA)
!Apa_T
aUKKiN0>>Rr`
`? ^*Kx1'E ăNj_{]:
.|66F{>W
.v䜨)aU_3
w
;ȯ4s]`}V 6民`Tl=~&}e$0m_vM:|^kU7Dd\y
{8e7}
z>
.(/wQ=
鶗M_G^@E?A^Ӵ=Մ@ɽ/MHmx].k
I\sOV;_W]}u{_~k[[VDGh%0I?cc8=x'0mO?o[}WU}}~¶l,qk5M{|4^FaK]-}o`qQ5vPokkZҵҵ ,!#U^^+m]hL{
+KJ+
cݭLu{Aa;m[J{t`\0A㏎*ӆM
2M46$ᅰU lqGDZ}m%m]aAh0]UPTS)ԃ-Wijzuzږ:w];A 82nCwv
~WTV\-2 N&ChHT.#w
b$܄]P)JI8wKR+HH8B(x٠a#GT"S2@ax н0M?"1d D).U3 ` i_s.舟:͌(pD(`y0<g ІjxN^[).-IT"H@aNa3f`a4wvzid`92
Dnqh`qwwiij_D DLO#vDI&q-z;M? ߭'!ވwr%1
O փN]>n__LrG "͠Aݿz|Nr"͜# N{zzz~|D%y:"e9\-d|_Ꞟn߭Y脇"cdcDL# nh0H A7jpa7O^
OL/y%t+Ol=<':N^W߿ꔂ.s/~-8ga~p~Uz}IIN~zo_66:C;n>k?+Do__
Hr r@?"nX7!qCv؞F?_ֿ
^b A-lփ|a0[CNC=t 4_6`??#G_}{[_U_a/s:_{/]҆
X066>OOtik=Wi+
Xa._Zik0_`⢘V*+ivkk^ip_5+K_5[T{aaW0,0$W5M5rp͐Ն⣃b%ح~tnmIk
Sh2'60K
tjC[Aa8`wiqe:A"h_^-aADe`B
0B""#j6mwkᐤ|0;L0Dt"
"""6]
; 5B-þh40Dtв CLaI8`"h0M4 в :paeQ4NN3Um
px!""6)BH_,3+AAם.Jo4kpX"OZuE=?MdC;d!2]'?___a4L\!9"w^jX7E!9{uOiouF2\dHe8R0Sa~3d`g@8A afa0>]W$8)&a3F0ypǂ h8楒")5# D `C4ޚ~Z2A*'* HhAӈ5aE
/ݑd>35Bh "`
T0:%GݝFD)Ų<\ 4BNONM;^myDBYnD{|0@O6`{& OxAaӴ+ӽUZ됖;3"VEh k&&G;4!|C ŧ__DKȗ&y Y%T==>~^i!uiz"CDd~n{OtK]>tޕV^wȘGȝgYz&۠zOOT\5v,BrXr%=ȹ=?ޟڧ骪vҽ/"&dN%D21ֈ"~EuA_pP>^ު~.z_GtMA=AAÌ&c ~mƭvB~#/~N_N_X`C`WD4_Ga
ꞪOU'.&O}Cp_/_7_odlp#uayh߉?\
^}K V')`_CדN/ A$d{66]}}A6<6ɎC8+_D%? ^
0ڧ ~?چoHz"}X@%н/__V^q~'yP>ؘh-z_}z_߯/@A?G^YFq(}5Nw_һ]IXk4,Wׯk!^FG^}?Uu]qQ{EG{]__[_j _LW _ 4_-}v0
X4.KkOJmXqIkaz|S{u~_cnK4~ǵUoᦙwA06[LSݾm5_w}ij0ړOuxi#hZ!Dm~Ӿ5Avd 骠i:(":D^۷~iڲ8Ga a?kaad+A#C0OD
l8'00@&Nc4B"""8"?!Pia44A:)2L!a0Dt""""""""""":rxk&?$_|Q'-NEMއ,YE`ə;-ӿB@UkBy;"N&j4Axت;I2
q 0Pa0A#")N@BZh03@Ӌ_v_ȁHrqsPAAЃ0|?g\)c5OA4@4OM?W)Dhإ8L)Cq"L qz ZiڮVDL"X2\G%AJ"0&-0|E-5O^CÓdEL\$Du"@xLB@ЈqhiuivrvH%#BC##!;7 &=릺zktxNC[M5
~Qw!/r%"cdn:j~_^u;AȖ# nBEȼ q}n]jּ[֓OMU=?oM2'H&Mүi'1C8r90}>Hl>BHxȗdGH!"%w&92"{ iz=:Mz6{9>GAD_A& /=k #>)>}=7֕tS{O;?N}vd%?_i맮/a/]?_^ _7IkS,D}UUoW7Aq...3__ת
~9䠭7Ң8!CO@c#,kwHϫ7Ft#43#%?]cx_
ёX_
ƿ~W~{"8v߯U_ACD?`NM7k_"[Zt֬&=B_$'Cxӧ[Ok|5Ճ 1Lj?jN#d;=|}VuEDf%zuv䇯
[*+8a?:>dypCkjkkkqqOU?ct~[]m[
ED=ijkvLz&:V}?V+[[ZXڶW\q
iWi15Lvk}m{K[`3X_~i}ݯ_]Xi/>8"STqL]OMv _VGa8aD{jm|m={N[?Jvam4CUq{VuM\׆d~ԆdXALLD"""?ikxMM["tjpdʨc:ikvONMݠ|0eRhGAݦ`xkky! hEt
m224=]@馚a8h80[P0dpC&SAv'P":*ڈ0[cǵCP¸`6s; ̩:gf r2DP]fKc0IYlM2];?pKgt4zJPxI~8'+3/Zd^#g&DS#怄e^W+G~D8!B'f(ytq B a0EC]}zqI 2
B-0BаrB0#qtI
`@D1 hZ!h>"OӻYm?vVRe8GF:AINhB,&L -~q]Sd""*)Hxz$*6 ~@7!ii'vv~ D"El4N5FaΡ@DP!B<Tia:N4iiҪwhȖ-&4D29tC -ca
@ppϙ1z @дhCz#9=4kzi!2%& ):AANzZa4-SUk'SNN}|_rCXr܉9y-
jT=~maO4cIHnD!1&=>77P_2%ׄT; ,|?BfB!2pL m
{ZM^_kk=J!GȏD̋ DL_]=i?^IҿG?z$>#x