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|Title: ||Combined use of LC–ESI-MS and antifungal tests for rapid identification of bioactive lipopeptides produced by Bacillus amyloliquefaciens CCMI 1051|
|Authors: ||Caldeira, A. Teresa|
|Keywords: ||Antimicrobial activity|
|Issue Date: ||27-May-2011|
|Citation: ||Caldeira A T, Arteiro J M, Coelho AV, Roseiro J C. (2011). “Combined use of LC–ESI-MS and antifungal tests for rapid identification of bioactive lipopeptides produced by Bacillus amyloliquefaciens CCMI 1051”. Process Biochemistry, 46 (9), pp. 1738-1746.|
|Abstract: ||The strain Bacillus amyloliquefaciens CCMI 1051 used in this study has been isolated in our laboratory from
healthy Quercus suber in the south of Portugal and shows high levels of antagonistic properties against
filamentous fungi that attack forest products industry due to the production of bioactive peptides.
A combined use of LC–ESI-MS and antifungal tests allowed a rapid identification of lipopeptides as
active compounds produced. Applying autobiographic methods it was possible to obtain active compounds.
LC–ESI-MS, a powerful tool for rapid identification, indicates the presence of lipopeptides and
MS2 electrospray ionization showed the partial sequence Tyr–Asn–Pro–Glu in the peptidic portion of
some compounds produced. The association of mass spectrometry and chromatography, used in parallel
with antifungal tests proved to be an efficient approach for the characterization of active lipopeptides
without the need of previous total isolation. This methodology can be employed for screening
and optimization the production of antifungal iturinic lipopeptides, showing a great potential for future
|Appears in Collections:||QUI - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica|
CQE - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica
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