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Title: cEpiderm, a Canine Skin Analog Suitable for In Vivo Testing Replacement
Authors: Marques, Mariana
Nunes, João
Ustymenko, Bárbara
Fialho, Luísa
Martins, Luís
Burke, Anthony
Filho, Cesar
Craveiro, Alexandre
Costa, Ana
Branco, Sandra
Antunes, Célia
Keywords: histotypical skin culture
skin analog
non-animal testing
Issue Date: 20-Oct-2022
Publisher: BioChem/MDPI
Citation: Marques, M.; Nunes, J.; Ustymenko, B.; Fialho, L.; Martins, L.; Burke, A.J.; Filho, C.; Craveiro, A.C.; Costa, A.R.; Branco, S.; et al. cEpiderm, a Canine Skin Analog Suitable for In Vivo Testing Replacement. BioChem 2022, 2, 215–220. biochem2040015
Abstract: Skin is one of the organs most tested for toxicity and safety evaluation during the process of drug research and development and in the past has usually been performed in vivo using animals. Over the last few years, non-animal alternatives have been developed and validated epidermis models for human and rat skin are already available. Our goal was to develop a histotypical canine skin analog, suitable for non-animal biocompatibility and biosafety assessment. Canine keratinocytes were seeded in an air-lift culture using an adapted version of the CELLnTEC protocol. Corrosion and irritation protocols were adapted from human EpiSkinTM. For histological analysis, sample biopsies were fixed in neutral-buffered formalin, and paraffin slices were routinely processed and stained with hematoxylin and eosin. A canine multilayer and stratified epidermal-like tissue (cEpiderm), confirmed by histological analysis, was obtained. The cEpiderm tissue exhibited normal morphological and functional characteristics of epidermis, namely impermeability and an adequate response to stressors. The cEpiderm is a promising canine skin model for non-animal safety testing of veterinary pharmaceuticals and/or cosmetics, significantly contributing to reducing undesirable in vivo approaches. cEpiderm is therefore a valid canine skin model and may be made commercially available either as a service or as a product.
Type: article
Appears in Collections:ICT - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica

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