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|Title: ||A simple method for labelling and detection of proteinaceous binders in art using fluorescent coumarin derivatives|
|Authors: ||Ooi, Su Yin|
Prates Ramalho, Joao P.
Caldeira, A. Teresa
|Keywords: ||identification of proteins|
proteins in easel paintings
|Issue Date: ||2019|
|Citation: ||Eur. Phys. J. Plus (2019) 134: 71|
|Abstract: ||The identification of protein binders used in easel paintings is an important information
to understand an artist’s technique and for making informed conservation and restoration decisions.
The present work presents a novel fluorescent labelling methodology, using a coumarin derivative
chromophore, C392STP (sodium (E/Z)-4-(4-(2-(6,7-dimethoxy-coumarin-3-yl) vinyl) benzoyl)-2,3,5,6-
tetrafluorobenzenesulfonate), as a fluorophore probe to bond proteinaceous binders used in paintings,
followed by separation and identification with protein patterns, by electrophoretic processes. Additionally,
theoretical quantum chemical calculations based on density functional theory (DFT) and time-dependent
density functional theory (TDDFT) have been performed in a coumarin derivative, which mimics the
coumarin bound to the proteins. The optimized methodology was tested on proteinaceous binder extracted from paint model micro-samples. The results evidence the applicability of this methodology as an
effective and useful analytical tool for the identification of protein binders obtained from easel paintings.|
|Appears in Collections:||CQE - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica|
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