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Title: A simple method for labelling and detection of proteinaceous binders in art using fluorescent coumarin derivatives
Authors: Ooi, Su Yin
Prates Ramalho, Joao P.
Pereira, Antonio
Martins, Sergio
Salvador, Catia
Caldeira, A. Teresa
Keywords: identification of proteins
proteins in easel paintings
fluorescent labelling
fluorescent coumarin
Issue Date: 2019
Citation: Eur. Phys. J. Plus (2019) 134: 71
Abstract: The identification of protein binders used in easel paintings is an important information to understand an artist’s technique and for making informed conservation and restoration decisions. The present work presents a novel fluorescent labelling methodology, using a coumarin derivative chromophore, C392STP (sodium (E/Z)-4-(4-(2-(6,7-dimethoxy-coumarin-3-yl) vinyl) benzoyl)-2,3,5,6- tetrafluorobenzenesulfonate), as a fluorophore probe to bond proteinaceous binders used in paintings, followed by separation and identification with protein patterns, by electrophoretic processes. Additionally, theoretical quantum chemical calculations based on density functional theory (DFT) and time-dependent density functional theory (TDDFT) have been performed in a coumarin derivative, which mimics the coumarin bound to the proteins. The optimized methodology was tested on proteinaceous binder extracted from paint model micro-samples. The results evidence the applicability of this methodology as an effective and useful analytical tool for the identification of protein binders obtained from easel paintings.
Type: article
Appears in Collections:CQE - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica

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