Please use this identifier to cite or link to this item: http://hdl.handle.net/10174/19660

Title: Wild carrot differentiation in Europe and positive selection at DcAOX1 gene
Authors: Nobre, T.
Oliveira, M
Arnholdt-Schmitt, B
Editors: Olsen, Kenneth M.
Keywords: oxidase (AOX) gene
DcAOX1
CWR hotspots of environment-adapted diversity
Issue Date: 21-Oct-2016
Publisher: PLoS One
Citation: Nobre T, Oliveira M, Arnholdt-Schmitt B (2016) Wild Carrot Differentiation in Europe and Selection at DcAOX1 Gene? PLoS ONE 11(10): e0164872. doi:10.1371/journal.pone.0164872
Abstract: By definition, the domestication process leads to an overall reduction of crop genetic diversity. This lead to the current search of genomic regions in wild crop relatives (CWR), an important task for modern carrot breeding. Nowadays massive sequencing possibilities can allow for discovery of novel genetic resources in wild populations, but this quest could be aided by the use of a surrogate gene (to first identify and prioritize novel wild populations for increased sequencing effort). Alternative oxidase (AOX) gene family seems to be linked to all kinds of abiotic and biotic stress reactions in various organisms and thus have the potential to be used in the identification of CWR hotspots of environment-adapted diversity. High variability of DcAOX1 was found in populations of wild carrot sampled across a West-European environmental gradient. Even though no direct relation was found with the analyzed climatic conditions or with physical distance, population differentiation exists and results mainly from the polymorphisms associated with DcAOX1 exon 1 and intron 1. The relatively high number of amino acid changes and the identification of several unusually variable positions (through a likelihood ratio test), suggests that DcAOX1 gene might be under positive selection. However, if positive selection is considered, it only acts on some specific populations (i.e. is in the form of adaptive differences in different population locations) given the observed high genetic diversity. We were able to identify two populations with higher levels of differentiation which are promising as hot spots of specific functional diversity.
URI: http://dx.doi.org/10.1371/journal.pone.0164872
http://hdl.handle.net/10174/19660
Type: article
Appears in Collections:CIMA - Publicações - Artigos em Revistas Internacionais Com Arbitragem Científica

Files in This Item:

File Description SizeFormat
Wild Carrot Differentiation in Europe and Selection at DcAOX1 Gene_.pdf6.91 MBAdobe PDFView/OpenRestrict Access. You can Request a copy!
FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpaceOrkut
Formato BibTex mendeley Endnote Logotipo do DeGóis 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Dspace Dspace
DSpace Software, version 1.6.2 Copyright © 2002-2008 MIT and Hewlett-Packard - Feedback
UEvora B-On Curriculum DeGois